Skin Safety Testing
- Identify substances that may cause reversible damage to the skin
- Reconstructed human epidermis (RhE) model, follows the OECD Test Guideline 439
- Cell viability measured via MTT assay post-exposure
- Supports classification under GHS and REACH
- Accepted alternative to animal testing for dermal irritation potential
- Evaluate irreversible damage to skin tissue caused by chemical exposure
- Same RhE models as irritation assay, follows the OECD Test Guideline 431
- Cell viability thresholds used to determine corrosive potential
- Regulatory classification of corrosive substances
- LuSens Assay (OECD TG 442D)
- Uses a luciferase-reporter gene in keratinocytes to detect activation of antioxidant response element (ARE)
- Indicates keratinocyte activation, a key event in the sensitization pathway
- Kinetic Direct Peptide Reactivity Assay (kDPRA, OECD TG 442C)
- Measures covalent binding of test substances to synthetic peptides over time
- Provides kinetic profiling for better discrimination of sensitizers
- Human Cell Line Activation Test (h-CLAT, OECD TG 442E)
- Uses THP-1 cells to detect expression of surface markers (CD86, CD54) indicative of dendritic cell activation
- Reflects immune system engagement in the sensitization process